2.1. System dependencies¶
This is known to install cleanly on most modern Linux systems with Python, Java, and some basic development libraries. On Ubuntu 14.04 LTS, the following APT packages should be installed on top of the vanilla setup:
python3 python3-pip python3-nose python-software-properties zlib zlib1g zlib1g-dev libblas3gf libblas-dev liblapack3gf liblapack-dev libatlas-dev libatlas3-base libatlas3gf-base libatlas-base-dev gfortran oracle-java8-installer libncurses5-dev
The Fortran libraries (including blas and atlas) are required to install numpy via pip from source. numpy is not actually required if you have Python 3.4, if you want to avoid this system dependency.
Java >= 1.7 is required by GATK and Picard.
2.2. Python dependencies¶
The command line tools require Python >= 2.7 or >= 3.4. Required packages (like pysam and Biopython) are listed in requirements.txt and can be installed the usual pip way:
pip install -r requirements.txt
Additionally, in order to use the pipeline infrastructure, Python 3.4 is required (Python 2 is not supported) and you must install snakemake as well:
pip install -r requirements-pipes.txt
However, most of the real functionality is encapsulated in the command line tools, which can be used without any of the pipeline infrastructure.
You should either sudo pip install or use a virtualenv (recommended).
2.3. Tool dependencies¶
A lot of effort has gone into writing auto download/compile wrappers for most of the bioinformatic tools we rely on here. They will auto-download and install the first time they are needed by any command. If you want to pre-install all of the external tools, simply type this:
python -m unittest test.test_tools.TestToolsInstallation -v
However, there are two tools in particular that cannot be auto-installed due to licensing restrictions. You will need to download and install these tools on your own (paying for it if your use case requires it) and set environment variables pointing to their installed location.
The environment variables you will need to set are GATK_PATH and NOVOALIGN_PATH. These should be set to the full directory path that contains these tools (the jar file for GATK and the executable binaries for Novoalign).
Alternatively, if you are using the Snakemake pipelines, you can create a dictionary called “env_vars” in the config.json file for Snakemake, and the pipelines will automatically set all environment variables prior to running any scripts.
The version of MOSAIK we use seems to fail compile on GCC-4.9 but compiles fine on GCC-4.4. We have not tried intermediate versions of GCC, nor the latest versions of MOSAIK.